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DETERMINATION OF MICROBIOLOGICAL SAFETY

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ABSTRACT
Determination of Microbiological safety of Ileogu Stream at Government Residential Area (GRA), Enugu was conducted.  Ten samples of the water from various parts were examined for total viable count for the presence of both paikogenic organisms and organisms of indicating organic matter pollution.  Also the same ten samples were used to detect coliform groups and Escherichia Coli, using presumptive coliform test, confirmatory test and completed test techniques.  
The mean total viable count for paikogenic organisms was 12cfu perml mean count for organisms indicating organic matter pollution was 16cfu per ml.  At presumptive test showed presence of Coliform groups in seven samples out of ten, the confirmatory test showed presence of both Coliform and Escherichia Coli in eight samples out of the ten samples.
Whereas the completed test showed mean count of Coliform group as 9 cfu per ml and mean count for Esterichia Coli as 16 cfu per ml.  The result showed that Ileogu Stream was polluted and also faecally contaminated.  Therefore this stream is not fit for drinking purpose.  Though can be used for washing clothes and bathing, since the microbial load was minimal.
TABLE OF CONTENTS
Title page
Certification page
Dedication
Acknowledgement
Abstract
Table of content
CHEATER   ONE
1.0 INTRODUCTION
1.1 Aim of the Study
1.2 Objective of the Study
1.3 Statement of Problem
1.4 Limitation of the Study
1.5 Hypothesis
1.6 Significance of the Study
CHAPTER   TWO
2.0 LITERATURE REVIEW
CHAPTER   THREE
3.0 MATERIALS AND METHODS
3.1 Materials
3.2 Methods
3.2.1 Sterilization
3.2.2 Collection of Water
3.2.3 Preparation of Culture
3.2.4 Plating Techniques for Water Analysis
CHAPTER   FOUR
4.0 RESULTS
CHAPTER   FIVE
5.0 DISCUSSION
CHAPTER   SIX
6.0 CONCLUSION AND RECOMMENDATION
REFERENCES

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